The five steps of protein making process are:
- Proteins are assembled at ribosomes.
- Proteins targeted for export to the cell membrane, or to specialized locations within the cell, complete their assembly on ribosomes bound to the rough endoplasmic reticulum.
- Newly assembled proteins are carried from the rough endoplasmic reticulum to the Golgi apparatus in vesicles.
- The Golgi apparatus further modifies proteins before sorting and packaging them in membrane-bound vesicles.
- Vesicles from the Golgi apparatus are shipped to their final destination in, or out of, the cell.