Illustration includes three pictures.
- Cutting DNA, captioned 'A restriction enzyme is like a key that fits only one lock. The EcoRI restriction enzyme can only recognize the base sequence GAATTC. It cuts each strand of DNA between the G and A bases, leaving single-stranded overhangs with the sequence AATT. The overhangs are called “sticky ends” because they can bond, or “stick,” to a DNA fragment with the complementary base sequence.'
- Separating DNA, captioned 'Gel electrophoresis is used to separate DNA fragments. After being cut by restriction enzymes, the fragments are put into wells on a gel that is similar to a slice of gelatin. An electric voltage moves them across the gel. Shorter fragments move faster than longer fragments. Within an hour or two, the fragments all separate, each appearing as a band on the gel.'
- Reading DNA, captioned 'A small proportion of dye-labeled nucleotides are used to make a complementary DNA strand. Each time a labeled nucleotide is added to the strand, DNA replication stops. Because each base was labeled with a different color, the result is color-coded DNA fragments of different lengths. When gel electrophoresis is used to separate the fragments, scientists can “read” the DNA sequence directly from the gel.'