How does the cell make RNA?
Cells invest large amounts of raw material and energy into making RNA molecules. Understanding how cells do this is essential to understanding how genes work.
Transcription Most of the work of making RNA takes place during transcription. In transcription, segments of DNA serve as templates to produce complementary RNA molecules. The base sequences of the transcribed RNA complement the base sequences of the template DNA.
In prokaryotes, RNA synthesis and protein synthesis take place in the cytoplasm. In eukaryotes, RNA is produced in the cell's nucleus and then moves to the cytoplasm to play a role in the production of protein. Our focus here is on transcription in eukaryotic cells.
Transcription requires an enzyme, known as RNA polymerase, that is similar to DNA polymerase. RNA polymerase binds to DNA during transcription and separates the DNA strands. It then uses one strand of DNA as a template from which to assemble nucleotides into a complementary strand of RNA, as shown in Figure 13–3. The ability to copy a single DNA sequence into RNA makes it possible for a single gene to produce hundreds or even thousands of RNA molecules.
FIGURE 13–3 Transcribing DNA into RNA During transcription, the enzyme RNA polymerase uses one strand of DNA as a template to assemble complementary nucleotides into a strand of RNA.
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