Quick Lab
GUIDED INQUIRY

Modeling Restriction Enzymes

1 Write a 50-base, double-stranded DNA sequence using the bases A, C, G, and T in random order. Include each sequence shown below at least once in the sequence you write.

2 Make three copies of your double-stranded sequence on three different-colored strips of paper.

3 Use the drawings below to see how the restriction enzyme EcoRI would cut your DNA sequence. Use scissors to cut one copy of the sequence as EcoRI would.

Diagram shows three DNA structures, which are cut (indicated by dotted lines) by three restriction enzymes: EcoRI, BamI, and HaeIII. The first two enzymes cut DNA in shape of a step and the last one cuts DNA vertically.

4 Use the procedure in Step 3 to cut apart another copy of your sequence as the restriction enzyme BamI would. Then, cut the third copy as the restriction enzyme HaeIII would.

5 Tape the single-stranded end of one of your DNA fragments to a complementary, single-stranded end of a classmate's fragment. This will form a single, double-stranded DNA molecule.

    Analyze and Conclude

  1. Observe Which restriction enzyme produced the most pieces? The fewest pieces?

  2. Evaluate How well did your model represent the actual process of using restriction enzymes to cut DNA? (Hint: Contrast the length of your model DNA sequence to the actual length of a DNA molecule.)

End ofPage 405

Table of Contents

Miller & Levine Biology
About the Authors
Changing the World of Biology Education
Contents
Diversity of Life: A Visual Guide DOL•1–DOL•64
Labs and Activities
Features
UNIT 1 The Nature of Life
1 The Science of Biology
2 The Chemistry of Life
Unit Project
UNIT 2 Ecology
3 The Biosphere
4 Ecosystems and Communities
5 Populations
6 Humans in the Biosphere
Unit Project
UNIT 3 Cells
7 Cell Structure and Function
8 Photosynthesis
9 Cellular Respiration and Fermentation
10 Cell Growth and Division
Unit Project
UNIT 4 Genetics
11 Introduction to Genetics
12 DNA
13 RNA and Protein Synthesis
14 Human Heredity
15 Genetic Engineering
Unit Project
UNIT 5 Evolution
16 Darwin's Theory of Evolution
17 Evolution of Populations
18 Classification
19 History of Life
Unit Project
UNIT 6 From Microorganisms to Plants
20 Viruses and Prokaryotes
21 Protists and Fungi
22 Introduction to Plants
23 Plant Structure and Function
24 Plant Reproduction and Response
Unit Project
UNIT 7 Animals
25 Introduction to Animals
26 Animal Evolution and Diversity
27 Animal Systems I
28 Animal Systems II
29 Animal Behavior
Unit Project
UNIT 8 The Human Body
30 Digestive and Excretory Systems
31 Nervous System
32 Skeletal, Muscular, and Integumentary Systems
33 Circulatory and Respiratory Systems
34 Endocrine and Reproductive Systems
35 Immune System and Disease
Unit Project
A Visual Guide to The Diversity of Life
HOW TO USE THIS GUIDE
THE TREE OF LIFE
Bacteria
Protists
Fungi
Plants
Animals
Appendices
Appendix A Science Skills
Appendix B Lab Skills
Appendix C Technology & Design
Appendix D Math Skills
Appendix E Periodic Table
Glossary
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q, R
S
T
U
V
W
X, Y, Z
Index
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q
R
S
T
U
V
W
X
Y
Z
Credits
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